Abstract
Purpose
The radioprotective effects of Dragon's blood (DB) and its extracts (DBE) were investigated
using the chromosomal aberrant test, micronucleus and oxidative stress assay for anti-clastogenic
and anti-oxidative activity.
Materials and methods
Adult BALB/C mice were exposed to the whole body irradiation with 4 Gy 60Co γ-rays. DB and DBE were administered orally once a day from 5 days prior to irradiation
treatment to 1 day after irradiation. The mice were sacrificed on 24 h after irradiation.
The cells of bone marrow were measured by counting different types of chromosomal
aberrations and the frequency of micronuclei. Oxidative stress response was carried
out by analysis of serum from blood.
Results
DB and DBE significantly decreased the number of bone marrow cells with chromosome
aberrations after irradiation with respect to irradiated alone group. The administration
of DB and DBE also significantly reduced the frequencies of micronucleated polychromatic
erythrocytes (MPCE) and micronucleated normochromatic erythrocytes (MNCE). In addition,
DB and DBE markedly increased the activity of antioxidant enzymes and the level of
antioxidant molecular. Malondialdehyde (MDA) and nitric oxide (NO) levels in serum
were significantly reduced by DB and DBE treatment.
Conclusions
Our data suggested that DB and DBE have potential radioprotective properties in mouse
bone marrow after 60Co γ-ray exposure, which support their candidature as a potential radioprotective
agent.
Keywords
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Article info
Publication history
Published online: December 20, 2013
Accepted:
December 4,
2013
Received in revised form:
November 19,
2013
Received:
September 12,
2013
Identification
Copyright
© 2013 Associazione Italiana di Fisica Medica. Published by Elsevier Inc. All rights reserved.
